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The two objectives of this study were: to determine Norwalk virus reduction by free chlorine, chlorine dioxide, and ozone in bench-scale disinfection experiments using quantitative RT-PCR for virus assays; and, to compare NV reductions to those of two other important health-related viruses, poliovirus type 1 (PV1) and coliphage MS2. Norwalk virus cannot be grown or assayed for infectivity in any known laboratory host, and therefore RT-PCR is the only sensitive and specific assay system available now. However, RT-PCR may not reliably quantify virus infectivity because the RNA of inactivated viruses may be amplified. Therefore, two other enteric viruses, poliovirus 1 (PV1) and coliphage MS2, were included in this study to compare RT-PCR assay data to infectivity assay data for those viruses and determine if they provide equivalent information. Also, these two viruses have been widely used as indicator viruses for disinfection efficiency. Product Details
Published: 01/01/1998 ISBN(s): 0898679796 Number of Pages: 16File Size: 1 file , 260 KB