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AWWA JAW6743

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AWWA JAW6743 Journal AWWA - New Approaches to Measuring Total Bacterial Populations in Water Supplies

Journal Article by American Water Works Association, 10/01/1982

Herson, Diane S.;Baker, Katherine H.

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This study compared the results of standard plate counts with those of the combined acridine orange (AO) and 2-(p- iodophenyl)-3-(pnitrophenyl)-5-phenyl-tetrazolium chloride (INT), or AOINT, technique for samples of distribution water and well water. To overcome the interference of a membrane filter's pores with the identification of stained cells, the authors used a gel-stripping technique to transfer the bacterial cells from membrane filters to a gelatin matrix before counts were made. Results show that the use of the AOINT technique provides a rapid and sensitive method for the determination of total numbers of metabolically active bacteria in a water sample, making it a useful adjunct to standard plating for monitoring problematic water supplies. In addition, water supply contamination can be detected in 4 hours using the AOINT technique. Includes 25 references, tables, figure.

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Edition: Vol. 74 - No. 10 Published: 10/01/1982 Number of Pages: 3File Size: 1 file , 1.2 MB